Re: Sanitizing water bottles using hydrogen peroxide

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Posted by Tony Mak on November 15, 2000 at 09:24:06:

In Reply to: Sanitizing water bottles using hydrogen peroxide posted by Mike Fafard on November 14, 2000 at 13:54:48:

Your question is frequently asked by many, unfortunately there is no straight answer to it. In Alberta, we have no regulation or standards regarding the use of H2O2 as an sanitizer for water bottles. However, here is some information that might help you to find the right approach specific to its application in your area.

First of all, regardless of the stock concentration, whether it is 35% or 6% or 3%, it means very little. Most importantly is the cocentration of the working solution. HP has been proven to be bactericidal even in concentrations as low as 0.003% (Yoshpe-Purer and Eyland, 1968)provided enough contact time. Now it is a good time to mention the efficiacy of a disinfectant/sanitizer is not only influenced by its concentration, but also contact time and temeprature to a lesse extent. So it is important to ask the question, "What is the contact time?" The same researchers have also reported that all suspensions were sterile "the following day". It is important to know that label claims on product bottles are often based on the Association of Official Analytical Chemists (AOAC) 10-min contact time protocol.

Secondly, the type and concentration of challenged organisms is important. Of course, we would assume that the water bottles are "clean". Yoshpe-Purer and Eyland (1968) also refported that at 90ppm, a 10(power 2)-10(power 3)suspension was "reduced" to a few cells in 5-6 hous; howeve, in 10(power 5) and 10(power 6)per ml suspensions, a similar contact time could only achieve a mere drop of about 2 log.

We should also be aware that hydrogen peroxide products are not all equal. While most studies, with a few exception, were based on pure HP, products in the market are hydrogen peroxide products. Many of them are called "stabilized accelerated hydrogen peroxide" with the addition of other chemicals to stabilize and to enhence their efficacies. It is important to be sure sanitizer to be used is food grade and is compatible with the target material.

Temperature is also important. Toledo et al. (1973) reported that for 25.8% HP, 99.99% deaction of B. subtilis could be achieved in 660 seconds at ambient temperature, but only require 30 seconds at 76 degree C.

The bactericidal, sporicidal, virucidal and fungicidal properties of HP are unequival (Turner, 1983; Ando and Tsuzuki, 1986; Bayliss and Waites, 1976, 1979, Toledo, 1973). However, with the many types of products available, and they are all chemically differnt (secret formulae?), there is no one single rule for concentration and efficacy correlation.

I would suggest that it is the responsibility of the proponent to prove the efficiacy of the "process". The specified process should be validated by a certified lab.

Good Luck!

Tony : A water bottling plant in Chilliwack is proposing to use a 35% food grade hydrogen peroxide solution at a concentration between 125-150ppm to sanitize used water bottles. Has anyone seen a regulation, standard or guideline for using hydrogen peroxide as a sanitizer? I have something from the US that says a concerntration of 550-1100 ppm is required for hydrogen peroxide.

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